Journal: Cancer medicine

Article Title: Molecular genetic and clinical characteristic analysis of primary signet ring cell carcinoma of urinary bladder identified by a novel OR2L5 mutation.

doi: 10.1002/cam4.5121

Figure Lengend Snippet: FIGURE 2 Images of the tumor cells from the primary signet ring cell carcinoma (SRCC) of the bladder (scale bar: 50 mm, 25 mm). The figures representative IHC images for CK7, CK20, and GATA-3 which are the marker of the primary SRCC of the bladder, Ki-67 proliferation index was 20%–30%, AB-PAS showed a low expression level. In addition, CK(AE1/AE2), EMA is the marker of epithelial tissue origin.

Article Snippet: At 4°C for 24 h, the slices were treated with various antibodies such as CK(AE1/AE2) (mouse monoclonal antipan cytokeratin (AE1/AE3) (dilution 1:200, A500- 019A, Invitrogen), EMA (Mouse Monoclonal anti- EMA/MUC1) (dilution 1:200; MA5- 11202; Invitrogen); CK7 (rabbit monoclonal anti- Cytokeratin 7) (dilution 1:1000, ab 68459, Abcam), CK20 (rabbit monoclonal anti- Cytokeratin 20) (dilution 1:200, ab76126, Abcam), GATA- 3 (rabbit monoclonal anti- GATA3) (dilution 1:500, ab 199428, Abcam), Ki67 (rabbit polyclonal anti- Ki67) (dilution 1:6000, 27,309- 1- AP, ProteinTech Group), CDX- 2 (rabbit monoclonal anti- CDX2) (dilution 1:1000, ab76541, Abcam), villin (rabbit monoclonal anti- Villin) (dilution 1:100, ab130751, Abcam), and β- catinin (rabbit monoclonal anti- beta Catenin) (dilution 1:500, ab130751, Abcam), ER (rabbit monoclonal antiestrogen Receptor alpha) (dilution 1:200, ab108398, Abcam), PR (rabbit monoclonal antiprogesterone Receptor) (dilution 1:400, ab16661, Abcam); PSA (rabbit polyclonal anti- PSA) (dilution 10 μg/ml, PA5- 119384, Invitrogen), and PSAP (rabbit polyclonal anti- PSAP) (dilution 1:400, PA582153, Invitrogen).

Techniques: Marker, Expressing

Journal: Developmental cell

Article Title: Pulmonary Neuroendocrine Cells Sense Succinate to Stimulate Myoepithelial Cell Contraction

doi: 10.1016/j.devcel.2022.08.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-KRT20 , Boster Bio , M02828; RRID: AB_2910267.

Techniques: Virus, Recombinant, Plasmid Preparation, Software

Journal: Molecular Cancer Research

Article Title: Crenolanib Regulates ERK and AKT/mTOR Signaling Pathways in RAS/BRAF-Mutated Colorectal Cancer Cells and Organoids

doi: 10.1158/1541-7786.mcr-20-0600

Figure Lengend Snippet: Figure 1. Gene mutation and expression analysis of crenolanib targeting molecules in clinical tumors. A, Manhattan plots of mutated genes from the TCGA database. Genes with more than 3% mutation are depicted on their chromosomal location. Names of genes with a high mutation rate are shown. B, Enriched pathways in AML and colorectal cancer. Pathways that were significantly enriched (P<0.05) are shown in AML. For colorectal cancer, only the top10 significantly enriched pathways are shown. C, mRNA expressions and mutations of crenolanib-targeting tyrosine kinase receptors. Gene expression levels in individual cases are depicted, and information on gene mutations are indicated by color. D, Immunohistochemical staining of PDGFRA, PDGFRB, and CK20 in colorectal cancer tissues; scale bars, 100 mm. E, A dimensionality reduction step using t-SNE. The analysis of t-SNE revealed a representative population that consists of colorectal cancer tissues, such as cancer cells, stromal cells, and white blood cells. F, The extracted cancer cells were shown. Cells expressing PDGFRA and PDGFRB are shownwithwarm or cold colors, corresponding to high or low expressions, respectively (N ¼ 5,000).

Article Snippet: After deparaffinization and blocking, sections were incubated with primary anti-CK20 rabbit monoclonal antibody (1:800, CST 13063, Cell Signaling Technology) and anti-PDGFRA mouse monoclonal antibody (25 mg/mL, MAB322–500, R&D systems) or anti-PDGFRB mouse monoclonal antibody (1:50, 610113, BD Transduction Laboratories) overnight at 4 C. Thereafter, the sections were probed with a secondary antibody (MACH 2 double stain polymer detection kit #2, Biocare Medical) for 30 minutes at ambient temperature.

Techniques: Mutagenesis, Expressing, Gene Expression, Immunohistochemical staining, Staining

Journal: CytoJournal

Article Title: Cytokeratin 20/p53 dual immunocytochemistry for improving the diagnostic accuracy of urine liquid-based cytology in the detection of urothelial neoplasm: A retrospective study

doi: 10.4103/cytojournal.cytojournal_23_17

Figure Lengend Snippet: (a) Papanicolaou smear of urine cytology sample showing atypical cells with multinucleation and vacuolated cytoplasm indicates umbrella cells. (b) Dual immunocytochemistry smear showing cytoplasmic red staining (CK20) without nuclear brown expression (p53) supports of umbrella cells.

Article Snippet: CK20 (monoclonal mouse, clone Ks 20.8; Diagnostic Biosystems, Pleasanton, CA, USA) and p53 (monoclonal mouse, clone DO-7; Dako A/S, Glostrup, Denmark) antibodies were used for DIC.

Techniques: Immunocytochemistry, Staining, Expressing

Journal: CytoJournal

Article Title: Cytokeratin 20/p53 dual immunocytochemistry for improving the diagnostic accuracy of urine liquid-based cytology in the detection of urothelial neoplasm: A retrospective study

doi: 10.4103/cytojournal.cytojournal_23_17

Figure Lengend Snippet: (a) A urine cytology sample showing a few clusters of atypical cells (Original cytologic diagnosis; suspicious) is seen. (b) Dual immunocytochemistry smear revealed cytoplasmic red (CK20) and nuclear brown (p53) indicates urothelial carcinoma cells.

Article Snippet: CK20 (monoclonal mouse, clone Ks 20.8; Diagnostic Biosystems, Pleasanton, CA, USA) and p53 (monoclonal mouse, clone DO-7; Dako A/S, Glostrup, Denmark) antibodies were used for DIC.

Techniques: Biomarker Discovery, Immunocytochemistry

Journal: CytoJournal

Article Title: Cytokeratin 20/p53 dual immunocytochemistry for improving the diagnostic accuracy of urine liquid-based cytology in the detection of urothelial neoplasm: A retrospective study

doi: 10.4103/cytojournal.cytojournal_23_17

Figure Lengend Snippet: (a) A urine cytology sample showing a few degenerated atypical cells (original cytologic diagnosis; highly suspicious). (b) Dual immunocytochemistry revealed CK20 only, histologically confirmed as bacillus Calmette–Guérin granulomatous cystitis.

Article Snippet: CK20 (monoclonal mouse, clone Ks 20.8; Diagnostic Biosystems, Pleasanton, CA, USA) and p53 (monoclonal mouse, clone DO-7; Dako A/S, Glostrup, Denmark) antibodies were used for DIC.

Techniques: Biomarker Discovery, Immunocytochemistry